Merhaba Misafir

Development and validation of a high-performance liquid chromatography–diode-array detection method for the determination of eight phenolic constituents in extracts of different wine species


Objectives: A new HPLC method was developed and validated for the determination of some phenolic compounds; gallic acid, chlorogenic acid, epigallocatechin, caffeic acid, vanillin, p-coumaric acid, rutin, and quercetin in some local wine and fruit wine samples. Materials and Methods: Analyses were performed on a Zorbax Eclipse C18 column (4.6 x 150 mm, 3.5-µm particle size) using a gradient system. Mobile phase A was a 10-mM phosphoric acid solution and mobile phase B was methanol using a flow rate of 1 mL/min. Phenolic components were monitored using a DAD at three different wavelengths. Results: The developed and validated method was generally linear between the 1-100 ppm concentration range. Recovery values were obtained in the range of 95-105% and repetitive. The method was successfully applied to investigate the phenolic profiles of different wine samples. Conclusion: As a result of the study, an accurate, sensitive and reliable HPLC-DAD method was developed. The method was successfully used to determine the concentrations of antioxidant phenolic constituents from some local wine extracts.

Yayınlandığı Kaynak : Turkish Journal of Pharmaceutical Sciences
  • Yıl : 2018
  • DOI : 10.4274/tjps.54154
  • Cilt : 15
  • ISSN : 1304-530X
  • Sayı : 1
  • eISSN : 2148-6247
  • Sayfa Aralığı : 22-28
  • IO Kayıt No : 68904
  • Yayıncı : Türk Eczacılar Birliği